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During the past two decades, 16S rRNA gene sequencing was used to characterize and compare the esophageal microbiomes (EMs) of healthy individuals with those in patients with esophageal diseases, including gastroesophageal reflux disease (GERD), Barrett’s esophagus (BE), esophageal adenocarcinoma (EAC), eosinophilic esophagitis (EoE), and esophageal motility disorders [1,2]. Analyzing the compositional differences between healthy and dysbiotic microbiomes in the esophagus has provided further insight into …