Engineered native E. coli (EcAZ)

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Here, we report the development of PROT3EcT, a suite of commensal Escherichia coli engineered to secrete proteins directly into their surroundings.
Lynch et al. 2023 Cell Host & Microbe 31(4): 634-649

These bacteria comprise three modular components: a modified bacterial protein secretion system, the associated regulatable transcriptional activator, and a secreted therapeutic payload.
Lynch et al. 2023 Cell Host & Microbe 31(4): 634-649

Gut native E. coli are genetically tractable and can serve as a chassis for transgene delivery (e.g., bile salt hydrolase) (Escherichia coli EcAZ-2 BSH)
Russell et al. 2022 Cell 185: 3263-3277

[Engineered native E. coli (Ec…

References (Sources)

Engineered Escherichia coli for the in situ secretion of therapeutic nanobodies in the gut
Lynch et al. 2023 Cell Host & Microbe 31(4): 634-649
Engineering the microbiome
Ainsworth 2020 Nature 577: S20
Gut microbiota: a new path to treat obesity
Muscogiuri et al. 2019 International Journal of Obesity Supplements 9: 10-19
Review
Intestinal transgene delivery with native E. coli chassis allows persistent physiological changes
Russell et al. 2022 Cell 185: 3263-3277
Reprogramming microbes to be pathogen-seeking killers
Hwang et al. 2014