Dendritic Cells (DCs) & MUCIN-1 (MUC1) / CA15-3

We have achieved stable high-level expression of a human tumor antigen, epithelial cell MUCIN-1 (MUC1) / CA15-3, on human dendritic cells (DCs) by retroviral transduction of CD34+ progenitor cells and their subsequent cytokine-induced differentiation into DCs. The process of retroviral transduction did not alter the growth or differentiation of DCs from CD34+ progenitor cells. Immunofluorescence and electron microscopy studies revealed that the expression of mucin was limited to the body of the DCs and was excluded from the cytoplasmic veils of the DCs. Furthermore, the expression of mucin on DCs was similar, if not identical, to the non-polarized expression of mucin found on carcinoma cells. In functional studies, …