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The use of inbred mouse strains and littermates, to remove genetic and microbial heterogeneity, respectively, are fundamental strategies that reduce intra-experimental variability.
The use of animals harboring the same starting microbiota might prove useful in minimizing microbiome-related confounding factors.
To this end, culture libraries based on human fecal isolates or complete communities can be utilized to define and manipulate microbiome activity in vitro and in gnotobiotic or human microbiome-associated (HMA) mouse models
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