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6-week-old C57BL/6N mice were fed (i) a conventional purified control diet (strong, low fiber; Puri-Starch), (ii) an alternative tailored purified control diet containing pectin and inulin (medium, high fiber; Puri-Fiber), or (iii) grain-based chow for 30 weeks.
Puri-Starch feeding resulted in significantly increased plasma insulin, cholesterol and transaminases, liver de novo lipogenesis and liver steatosis, and altered gut microbiota composition compared to chow-fed mice.
In contrast, Puri-Fiber had little effect on systemic parameters and liv…