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The very costly shotgun metagenomics (DNA, Genome level), now widely applied as untargeted next-generation-sequencing in microbiome studies, examines the microbial sample's entire DNA. This technique involves fragmentation and random sequencing of DNA from the collection of genomes and genes within the sample, and the use of advanced bioinformatic techniques to analyse the sequencing data obtained
It provides categories of genes (COGs) and valuable functional information down to the strain level and all microorganism types (including archaea, fungi, and viruses). It also provides information about what thei…