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A key enzyme in Escherichia coli (E. coli) is purine nucleoside phosphorylase (PNP), which plays a significant role in purine metabolism. PNP catalyzes the phosphorolysis of purine nucleosides, such as inosine and guanosine, resulting in the formation of purine bases like hypoxanthine or guanine and ribose-1-phosphate. This enzyme is essential in the nucleoside salvage pathway, allowing Escherichia coli (E. coli) to efficient…