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i-ROBOT refers to a new class of engineered probiotic bacteria designed for real-time, in situ diagnosis and therapy of diseases, particularly inflammatory bowel disease (IBD). These bacteria are created using advanced synthetic biology techniques to autonomously sense disease biomarkers, record disease states, and deliver therapeutic agents directly at the site of inflammation. For Inflammatory Bowel Disease (IBD), the bacteria sense thiosulfate, a marker of gut inflammation. In response to inflammation, i-ROBOT releases a therape…